A study of effect of acute and chronic administration of aqueous extract of Calotropis procera leaves on maximal electroshock induced seizures in rats

Authors

  • Megha Madhyastha Clinical Data Coordinator, Quintiles Research India Pvt Ltd., Bangalore, Karnataka 576104, India
  • Smita Shenoy Department of Pharmacology, Kasturba Medical College, Manipal, Manipal University, Karnataka 576104, India
  • Vagdevi Hangarakatte Ramachandra Department of Pharmacology, Subbaiah Institute of Medical Sciences, Shimoga, Karnataka, India
  • Rajappayya Desai Department of Pharmacology, Gadag Institute of Medical Sciences, Gadag, Karnataka, India
  • Amod Tilak Department of Pharmacology, Kasturba Medical College, Manipal, Manipal University, Karnataka 576104, India
  • Meena Kumari Department of Pharmacology, Kasturba Medical College, Manipal, Manipal University, Karnataka 576104, India
  • Manjunath Shetty Department of Pharmacology, Melaka Manipal Medical College, Manipal, Karnataka 576104, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20164410

Keywords:

Tonic hind limb extension, Phenytoin

Abstract

Background: The effect of acute and chronic administration of aqueous extract of leaves of Calotropis procera on maximal electroshock (MES) induced seizures in rats was evaluated.

Methods: Four groups of wistar rats were treated with distilled water 10 mL/kg (oral), phenytoin 25 mg/kg (intraperitoneal), 250mg/kg (oral) and 500mg/kg (oral) of aqueous extract of leaves of C procera, respectively in both acute and chronic studies. For producing maximal electroshock seizures, an electroconvulsiometer was used to deliver an electric stimulus of 150mA for 0.2 sec. The duration of hind limb extension (HLE) and seizures were noted in seconds (sec). In the acute study, the shock was given 1 h after administration of drugs. For the chronic study, all drugs were administered for 6 weeks. One hour after the last dose of drugs, the shock was administered. Data was analyzed by one way ANOVA followed by Tukey’s post hoc test.

Results: There was a significant decrease in the duration of hind limb extension, and convulsions as compared to control following acute (p<0.01 vs control) and chronic (p<0.05 vs control) administration of aqueous extract of leaves of Calotropis procera in rats.

Conclusions: The study demonstrated that aqueous extract of C procera leaves resulted in significant protection against maximal electroshock induced seizures in rats.

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Published

2016-12-24

How to Cite

Madhyastha, M., Shenoy, S., Ramachandra, V. H., Desai, R., Tilak, A., Kumari, M., & Shetty, M. (2016). A study of effect of acute and chronic administration of aqueous extract of Calotropis procera leaves on maximal electroshock induced seizures in rats. International Journal of Basic & Clinical Pharmacology, 6(1), 1–4. https://doi.org/10.18203/2319-2003.ijbcp20164410

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