DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20210552

Study of anti-inflammatory activity of Ficus racemosa linn stem bark extract in albino rats

Priya Mohan B. N., Suma Jayaram, Sanitha Kuriachan

Abstract


Background: Inflammation is part of the complex biological response of vascular tissues to harmful stimuli. Though there are standard anti-inflammatory drugs like aspirin, diclofenac, etc., these are not free of side effects. This has led to an increase in demand for natural products with anti-inflammatory activity having less side effects. Hence the study was conducted to evaluate the anti-inflammatory activity of ethanolic extract of Ficus racemose (EEFR) in albino rats.

Methods: Healthy albino rats of either sex were divided into 4 groups of 6 animals each. Group1-control, group 2-diclofenac sodium 2 mg/kg and group 3 and 4 EEFR (200 and 400 mg/kg respectively), anti-inflammatory activity was evaluated by Carrageenan induced paw oedema: formalin induced-peritonitis and cotton pellet induced granuloma model for in vivo activity and protein denaturation test for in vitro activity.

Results: EEFR exhibited significant in vitro (p<0.001) anti-inflammatory effect at the dose of 200 and 400 mg/kg. EEFR produced 61.37% inhibition at the dose of 400 mg/kg and diclofenac (standard drug) produced 62.95% of inhibition after 3 hours of drug treatment in carrageenan induced paw oedema. The exudate volume was decreased in formalin induced peritonitis by EEFR and diclofenac significantly (p<0.001). In cotton pellet induced granuloma EEFR (400 mg/kg) and diclofenac showed decreased formation of granuloma by 28.36% and 28.00% (p<0.001) respectively.

Conclusions: EEFR has significant anti-inflammatory activity in both acute and chronic model in a dose dependant manner in comparison with standard drug.


Keywords


Anti-Inflammatory agents, Carrageenan, Diclofenac, Edema, Ficus, Granuloma, Peritonitis

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References


Abbas AB, Lichtman AH. In Innate Immunity, ch. 2. In Saunders (Elsevier). Basic Immunology. Functions and disorders of the immune system. 2009;3.

Sosa S, Balicet MJ, Arvigo R, Esposito RG, Pizza C, Altinier GA et al. Screening of the topical anti-inflammatory activity of some Central American plants. J. Ethnopharmacol. 2002;8:211-5.

Jay AN, Jagir P, Semuel MR, Shabaraya AR. Anti-inflammatory activity of fractionated extracts of Achyranthes aspera Linn leaves. J Appl Pharmace Sci. 2011;1(8):188-90.

Patil VV, Pimprikar RB, Sutar NG. Anti-Hyperglycemic activity of leaves. J Pharm Res 2009;2:54-57.

Deep P, Singh AK, Md. Ansari T, Raghav P. Pharmacological Potentials of Ficus racemosa-A Review. Int J Pharm Sci Rev Res. 2013;22(1):29-34.

Rageeb M, Usman M, Rohit P. Medicinal uses of Ficus racemosa linn. Int J Pharmac Arch. 2013;2(3):33-42.

Gowda SKP, Swamy VBM. Protective effect of stem bark ethanol and aqueous extracts of Ficus racemosa against cisplatin induced nephrotoxicity in mice. Int J Res Pharm Chem. 2011;1(3);465-9.

Prasanna Krishna V, Sunil V, Venugopal Johnson P. Evaluation of Acute and Chronic Toxicity Studies of Ethanolic Extract Of Ficus Glomerata L. Int J Pharmac Develop Technol. 2011;1(2):66-70.

Mallikadevi T, Paulsamy S, Karthika K, Jamuna S. In Vitro And In Vivo Anti-Inflammatory Activity Of Whole Plant Methanolic Extract Of Mukia Maderaspatana (L.) M. Roem. (Cucurbitaceae) Int J Pharm Pharm Sci. 2012;4(5):435-9.

Gerhard Vogel H. Analgesic, Anti-Inflammatory, and Anti-Pyretic Activity. Drug Discovery and Evaluation: Pharmacological Assays. Springer-Verlag Berlin Heidelberg New York. 2008;3:1047113.

Bhuvana KB, Hema NG. An evaluation of aloe vera for its acute anti-inflammatory activity in rat peritonitis model. World J Pharmac Res. 2014;3(3):4508-19.

Schmid-Schönbein W. Analysis of inflammation. Annu Rev Biomed Eng. 2006;8:93-151.

Brenner PS, Krakauer T. Regulation of inflammation: A review of recent advances in anti-inflammatory strategies. Curr Med Chem Anti-Inflamm Anti-Allergy Agents. 2003;2:274-83.

Hansson GK. Inflammation, atherosclerosis, and coronary artery disease. N Engl J Med. 2005;352:1685-95.

Hotamisligil GS. Inflammation and metabolic disorders. Nature. 2006;444:860-7.

Tarzami ST. Chemokines and inflammation in heart disease: Adaptive or maladaptive? Int J Clin Exp Med. 2011;4:74-80.

Watanabe T, Higuchi K, Tanigawa T, Tominaga K, Fujiwara Y, Arakawa T. Mechanisms of peptic ulcer recurrence: role of inflammation. Inflammo pharmacol. 2002;10:291-302.

Smith GR, Missailidis S. Cancer, inflammation and the AT1 and AT2 receptors. J. Inflamm. 2004;1:1-12.

Fosslien E. Review: Cardiovascular complications of non-steroidal anti-inflammatory drugs. Ann Clin Lab Sci. 2005;35:347-85.

Pereira LP, Da Silva RO, Ringel PHSF, Da Silva KES, Assreuy AMS, Pereira MG. Polysaccharides fractions of Caesalpinia ferrea pods: Potential anti-inflammatory usage. J Ethnopharmacol. 2012;139:642-8.

De Brito TV, Prudêncio RS, Sales AB, Júnior FCV, Candeira SJN, Franco AX et al. Anti-inflammatory effect of a sulphated polysaccharide fraction extracted from the red algae Hypnea musciformis via the supression of neutrophil migration by the nitric oxide signalling pathway. J Pharm Pharmacol. 2013; 65:724-33.

Mandal SC, Maity TK, Das J, Saha BP, Pal M, Anti-inflammatory evaluation of Ficus Racemosa Linn Leaf extract, J Ethnopharmacol. 2000;72(1-2):87-92.

Forestieri AM, Monfotre MT, Ragusa S, Trovato A, Lauk L, Anti-inflammatory, analgesic and antipyretic activity in rodents of plant extracts used in Africans Medicine. Phytother Res. 1996;10(2):100-3.

Li RW, Leach DN, Myers SP, Lin GD, Leach GJ, Waterman PG. A new anti-inflammatory glucoside from Ficus Racemosa Linn. Planta Med. 2004;70(5):421-6.

Opie EL. On the relation of necrosis and inflammation to denaturation of proteins. J Exp Med. 1962;115:597-608.

Umapathy E, Ndebia EJ, Meeme A, Adam B, Menziwa P, Nkeh-Chungag BN et al. An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation. J Med Plants Res. 2010;4:789-95.

Sunil LH, Priyanka SH. Evaluation of analgesic and anti-inflammatory activity of Ficus racemosa Linn. stem bark extract in rats and mice. Pharmacogosy J. 2010;2(6)65-70.

Mehtaa DS, Katariab BC, Chhaiyaa SB. Wound healing and anti-inflammatory activity of extract of Ficus racemosa linn. bark in albino rats. Int J Basic Clin Pharmacol. 2012;1(2):111-5.

Crunkhorn P, Meacock SC. Mediators of the inflammation induced in the rat paw by carrageenin. Bri J Pharmacol. 1971;42(3):392-402.

Vane J, Booting R. Inflammation and mechanism of action of anti-inflammatory drugs. FASEB J. 1987;1:89-96.

Nivsarkar M, Mukherjee M, Patel M, Padh H, Bapu C. Launaea nudicaulis leaf juice exhibits anti-inflammatory action in acute and chronic inflammation models in rats. Indian Drugs. 2002;39:290.

Vinegar R, Schreiber W, Hugo R. Biphasic development of carrageenin edema in rats. J Pharmacol Exp Ther. 1969;166:96-103.

Tonussi CR, Ferreira SH. Tumour necrosis factor-α mediates carrageenin-induced knee-joint incapacitation and also triggers overt nociception in previously inflamed rat knee-joints. Pain. 1999;82(1):81-7.

Damas J, Liégeois JF. The inflammatory reaction induced by formalin in the rat paw. Naunyn Schmiedebergs Arch Pharmacol. 1999;359(3):220-7.

Arrigoni-Martelli E. Inflammation and Anti-inflammatories. New York: Spectrum Publication Inc. 1977;119-20.

Swingle KF, Shideman FE. Phases of the inflammatory response to subcutaneous implantation of a cotton pellet and their modification by certain anti-inflammatory agents. J Pharmacl Exp Therap. 1972;13:226-34.

Babu P, Pandikumar P, Ignacimuthu S. Anti-inflammatory activity of Albizia lebbeck Benth., an ethomedicinal plant, in acute and chronic animal models of inflammation. J Ethnopharmacol. 2009;125:356-60.