DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20221594

Antiplasmodial activity of the ethanol extract of Dacryodes edulis leaf in Plasmodium berghei infected mice

Chikezie N. Okwesileze, Godwin C. Akuodor, Casimir C. Ofor, Daniel O. J. Aja, Gideon A. Ibiam, Cajetan E. Ilo

Abstract


Background: Malaria treatment in Africa is increasingly becoming difficult resulting from Plasmodium falciparum resistant to available antimalarial agents. Therefore, evaluating plants used in traditional medicines can lead to alternative treatment against malaria.

Methods: This study evaluated the antiplasmodial activity of ethanol extract of Dacryodes edulis in chloroquine sensitive Plasmodium berghei NK65 strain in mice. The 4-day suppressive test, repository and curative effect against established rodent malaria infection models were assayed in this study. The oral acute toxicity test and phytochemical screening were also carried out on the ethanol leaf extract.

Results: The ethanol leaf extract of Dacryodes edulis (D. edulis) at varying doses of 100 mg/kg, 200 mg/kg, 400 mg/kg significantly (p<0.05) exhibited dose-dependent decrease in parasitaemia at suppressive, repository and curative studies. Chloroquine 10 mg/kg significantly (p<0.05) decreased parasitaemia levels in the three models of malaria infections. The antiplasmodial activity of the leaf extract is comparable with the standard drug. The extract was found to be non-toxic acutely in mice.

Conclusions: The findings show that D. edulis ethanol leaf extract demonstrated high antiplasmodial activity in a dose-dependent fashion. Thus, supporting claims of the plant traditional therapeutic importance for malaria treatment, and can be developed as an alternative therapy against the disease.


Keywords


Dacryodes edulis, Leaf extract, Herbal medicine, Antiplasmodial activity, Mice

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References


Ritter JM, Lewis LD, Mant TGK, Ferro A. A textbook of Clinical Pharmacology and Therapeutics, 5th Edition, Hodden Education Publisher, London. 2008;364.

de Ridder S, van der Kooy F, Verpote R. Artemisia annua as a self-reliant treatment for Malaria in developing countries. J Ethnopharmacol. 2008;120:303-14.

World Health Organization Malaria. 2012. Fact sheet no. 94. Available at: http://www.who.int/mediacentre/ factsheets/fs094/en/. Accessed on 05 February 2022.

Plowe CV, Djimde A, Bouare M, Doumbo O, Wellens TE. Pyrimethamine and proguanil resistance-conferring mutation in Plasmodium falciparum dihydrofolate reductase: polymerase chain reaction methods for surveillance in Africa. Am J Trop Med Hyg. 1995;52:565-68.

Kaushik NK, Bagavan A, Rahuman AA, Mohanakrishna D, Kamaraj C, Elango G. Antiplasmodial potential of selected medicinal plants from Eastern Ghats of South India. Exp Parasitol. 2013;134:26-62.

Lombardino JG, Lowe JA. The role of the medicinal chemist in drug discovery-then and now. Nat Rev Drug Discov. 2004;3:853-62.

Akuodor GC, Ajoku GA, Ezeunala MN, Chilaka KC Asika EC. Antimalarial potential of the ethanolic leaf extract of Pseudocedrala kotschyi. J Acute Disease. 2015;23-7.

Akuodor GC, Ezeonu TC, Essien AD, Asika EC, Chilaka KC Nwachukwu DC, et al. In vivo antiplasmodial activity of Bombax buonopozense root bark aqueous extract in infected by Plasmodium berghei. J Tradit Chin Med. 2017;37(4):431-5.

Aidu M, Erhabor JO, Towuru GE. Antimicrobial effects of the chloroform and ethanolic leaf extracts of Dacryodes edulis (G. Don) H. J. Lam, Garcinia Kola Heckel and Chrysophyllum Albidum G. Don on some test isolates. Med Sci. 2013;1(3):63-6.

Leakey RRB, Tchoundjeu Z, Schreckenberg K, Schacleton SE, Shackleton CM. Agroforestry tree products (AFTPS): Targeting poverty and enhanced livelihoods. Int J Agric Sustainability. 2005;3(1):1-23.

Isaac IO, Ekpa OD. Minerals and anti-nutrients in two varieties of African pear (Dacryodes edulis). J. Food Technology. 2009;7:106-10.

Iyawe HOT, Osagie VE, Esekheigbe A and Azih MC. Nutrient composition and effect of seed extracts of Africa black pear (Dacyodes edulis) on rats. Asian J Plant Science. 2007;6:878-80.

Kengue J, Tchuenguem F, Adewusi H. Towards the improvement of safou (Dacryodes edulis), population and reproduction Biology. Afri J Biotec. 2002;12:73-84.

Nwokeji E, Nwufo M, Ibe A. Investigation on the uses and fungal diseases of African pear (Dacryodes edulis) in Imo state, Nigeria. Nig J Plant Protect. 2005;22:9-16.

Omonhinmin A. Ethnobotany of Dacryodes edulis (G. Don) in Southern Nigeria 2: Practices and application among Igbo speaking people. J Ethnobotany Res Application. 2012;10:175-84.

Egharvba RKA, IKhatua I. Ethno medical uses of plants in the treatment of various skin diseases in Ovia North Easth, Edo State Nig Res J Agric Biol Sci. 2008;4(1):58-64.

Okwu D, Nnamdi F. Evaluation of the chemical composition of Dacryodes edulis and Raphia hookeri Mann and Wendl exudates used in Herbal medicine in South East Nigeria. Afri J Tradit Complem Altern Med. 2008;5(2):194-200.

Obame L, Edou P, Bassole I, Koudou J, Agnaniet H, Eba F, Traore A. Chemical composition, antioxidant, antimicrobial properties of essential oil of Dacryodes edulis from Gabon. Afri J Microbiol Res. 2008;2:148-52.

Nguefack EC. Hypoglycemic, Hypolipidemic and antioxidant activity of some Cameroonian Medicinal plants. Lyon France. 2009;49.

Agbor G, Kuate D, Oben J. Medicinal plants can be good sources of antioxidants, case study in Cameroon. Park J Biological Sci. 2007;100:537-44.

Mpiana P, Tshibangu D, Shetonde O, Ngboluak. In Vitro antidrepanocytary activity (anti-sickle cell anaemia) of some Congolese plants. Phytomedicine. 2007;14:192-5.

Billmary C, Janne R, Luccro M, Maria C. Preliminary Phytochemical Screening of Pirmenta racemosa Var. racemose (myrtaceae) from Tachira-venezula. Pharmacology online. 2015;1:121-5.

Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol. 1983;54:275-87.

NIH. Guide for the Care and Use of Laboratory Animals. 8th ed. Bethesda MD. National Institutes of Health. 2011;82-3.

Akuodor G, Amos G, Essien A, David-oku E. Akpan J, Ezeokpo B. Antimalaria potency of the leaf extract of Aspilia Africana (Pears). Asian Pac J Trop Med. 2012;5:126-9.

Essien AD, Essiet GA, Akuodor GC, Nwoboo NN, Akpan JL, Utsalo SJ. Evaluation of anti plasmochial effect of the ethanolic leaf extract of Salacia lehmbachii on Plasmodium berghei infected mice. Eur J Biol Res. 2017;7(2):124-30.

Abdulrazak N, Asiya UI, Usman NS, Unata IM, Farida A. Antiplasmodial activity of ethanolic extract of root and stem back of Cassia sieberiana DC on mice. J. Intercult Ethnopharm. 2015;4(2):96-101.

Builder MI, Wannang NN, Ajoku GA, Builder PF, Orisadipe A, Aguiyi JC. Evaluation of the antiplasmodial potential of Vernonia ambigua kotschu and Peyr(Asteraceae). Int J Pharmacol. 2011;7:238-47.

Mbah CC, Akuodor GC, Anyalewechi NA, Iwuanyanwu TC, Osunkwo UA. In vivo antiplasmodial activities of aqueous extract of Bridelia ferruginea stem bark against Plasmodium berghei berghei in mice. Pharm Biol. 2012;50:188-92.