Published: 2020-08-25

The antianxiety activity of aqueous extract of Withania coagulans fruits in swiss albino mice by elevated plus maze

Devesh D. Gosavi, Amit S. Kamdi, Suvarna M. Kalambe, Pankaj N. Bohra


Background: Several anti-anxiety drugs used today have the numerous side effects. The Withania coagulans a rare species, is not studied much for its actions on anxiety except in late seventies where it was explored for neuropsychopharmacology. Thus, it was thought commendable to explore the anti-anxiety activities of aqueous extract of Withania coagulans fruits in swiss albino mice using elevated plus maze test.

Methods: Elevated plus maze (EPM) test was used for evaluating the anti-anxiety action of Withania coagulans fruits’ aqueous extract. If the extract had anti-anxiety action, then it was expected that the time spent in the open space would increase and that in the closed space would decrease. This increase in time in the open space, if found statistically significant, was considered for antianxiety action.

Results: There was statistically highly significant (p value <0.001) association observed between the aqueous extract of Withania coagulans fruits with antianxiety action in the swiss albino mice by elevated plus maze test.

Conclusions: The aqueous extract of Withania coagulans fruits did prove the antianxiety effect in the swiss albino mice by elevated plus maze test.


Anti-anxiety, Swiss albino mice, Withania coagulans, Elevated plus maze

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Steimer T. The biology of fear and anxiety related behaviors. Dialogues Clin Neurosci. 2002;4(3):231.

Reddy VM, Chandrashekar CR. Prevalence of mental and behavioural disorders in India: a meta-analysis. Indian J Psychiatry. 1998;40(2):149.

Tiwari SC, Srivastava S. Geropsychiatric morbidity in rural uttar pradesh. Indian J Psychiatry. 1998;40(3):266-73.

World Health Organization (WHO). World Health Organization Manual of the international statistical classification of diseases, injuries and causes of death, ninth revision. Geneva, Switz; 1992.

Murthy. National mental health survey of India 2015-2016. Indian J Psychiatry. 2017;59(1):21.

Tylee A, Walters P. Onset of action of antidepressants. BMJ. 2007;334(7600):911-2.

Pandey I, Nama KS. Withania Coagulans (stocks) Dunal: A Rare Ethnomedicinal Plant of the Western Rajasthan Desert. IJPBR. 2015;2(2):34-40.

Pandey RP, Meena SL, Padhye PM, Singhadiya MK. A review of depleting plant resources, their present status and conservation in Rajasthan, India. In: Biological Forum. Satya Prakashan; 2012:213-230.

Budhiraja RD, Bala S, Garg KN. Pharmacological investigations on fruits of Withania coagulans, Dunal. Planta Med. 1977;32(2):154-7.

Qasim S, Zafar A, Saif MS, Ali Z, Nazar M, Waqas M, et al. Green synthesis of iron oxide nanorods using Withania Coagulans extract improved photocatalytic degradation and antimicrobial activity. J Photochem Photobiol B Biol. 2020;111784.

Lateef T, Qureshi SA. Centratherum anthelminticum and Withania coagulans improves lipid profile and oxidative stress in triton X-100 induced hyperlipidemic rabbits. Group. 2020;1(11.36):1-22.

Ani AOA, Bazzaz AA. Glycated low-density lipoprotein in diabetic and non-diabetic patients. Asian J Pharm Clin Res. 2020;13(2):123-6.

Meeran SB, Subburaya U, Narasimhan G. In Silico and In Vitro Screening of Ethanolic Extract of Fruits of Withania Coagulans against Diabetes. Res J Pharm Technol. 2020;13(2):631-5.

Pellow S, Chopin P, File SE, Briley M. Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods. 1985;14(3):149-67.

Rodgers RJ, Dalvi A. Anxiety, defence and the elevated plus-maze. In: Neuroscience and Biobehavioral Reviews; 1997:801-810.

Korte SM, Boer DSF. A robust animal model of state anxiety: fear-potentiated behaviour in the elevated plus-maze. Eur J Pharmacol. 2003;463(1):163-75.

Guideline OO. 425: Acute oral toxicity up and down procedure. OECD Guidel Test Chem Organ Econ Coop Dev Paris; 2001.

Soxhlet F. Die gewichtsanalytische bestimmung des milchfettes. Polytech J. 1879;232:461-5.

Komada M, Takao K, Miyakawa T. Elevated plus maze for mice. Journal Vis Exp. 2008;(22):1088-8.

Bhattacharya1 SK, Bhattacharya A, Sairam K, Ghosaf S. Anxiolytic-antidepressant activity of Withania somnifera glycowithanolides: an experimental study. Phytomedicine. 2000;7(6):463-9.

Sood A, Kumar A, Dhawan DK, Sandhir R. Propensity of Withania somnifera to Attenuate Behavioural, Biochemical, and Histological Alterations in Experimental Model of Stroke. Cell Mol Neurobiol. 2015:1-16.

Singh N, Bhalla M, Jager DP, Gilca M. An overview on Ashwagandha: A Rasayana (Rejuvenator) of Ayurveda. African J Tradit Complement Altern Med. 2011;8(5):208-13.

Maurya R. Chemistry and pharmacology of Withania coagulans: an Ayurvedic remedy. J Pharm Pharmacol. 2010;62(2):153-60.

Maguire JL, Stell BM, Rafizadeh M, Mody I. Ovarian cycle-linked changes in GABA(A) receptors mediating tonic inhibition alter seizure susceptibility and anxiety. Nat Neurosci. 2005;8(6):797-804.

Koonce CJ, Walf AA, Frye CA. Type 1 5α-reductase may be required for estrous cycle changes in affective behaviors of female mice. Behav Brain Res. 2012;226(2):376-80.

Liu A, Papale AE, Hengenius J, Patel K, Ermentrout B, Urban NN. Mouse navigation strategies for odor source localization. Front Neurosci. 2020;14.

Hogg S. A review of the validity and variability of the elevated plus-maze as an animal model of anxiety. Pharmacol Biochem Behav. 1996;54(1):21-30.