DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20200542

Evaluation of antioxidant potential of melatonin in periodontitis with a focus on vitamin C

Anagha P. Marawar, Pramod P. Marawar, D. H. Nandal, Rahul R. Kunkulol

Abstract


Background: The present study was done with an objective to evaluate the effect of melatonin as an antioxidant, with a focus on vitamin C, in patients suffering from periodontitis.

Methods: This study was conducted jointly in the Department of Periodontics, Rural Dental College, and Department of Pharmacology, PIMS, Loni. Patients suffering from chronic periodontitis, of age between 18 to 65 years of either gender were included in the study, after obtaining a written informed consent. Patients were divided into three Groups. Group A included patients who underwent scaling and root planning (SRP) alone, Group B included patients who underwent SRP and supplemented with vitamin E 200 IU daily at night for 4 weeks. Group C included patients who underwent SRP and supplemented with tablet melatonin 3 mg daily at night for 4 weeks. Estimation of vitamin C was done on day 0, day 30, day 60 and day 90.

Results: A total 240 patients were enrolled in the study, 80 in each group. Statistical analysis was done using ‘Z’ test of difference between two sample means for comparison of vitamin C among the three groups. It was observed that, there was a highly significant increase in mean values of vitamin C in group C when compared with group A and group B at 1st, 2nd and 3rd visits (p<0.01).

Conclusions: Melatonin acts as an antioxidant in the patients of periodontitis which has positive effect on biochemical parameters of periodontitis, conferring a new facet to the management of periodontitis and an attempt to impede the disease progression.


Keywords


Antioxidant, Melatonin, Periodontitis, Vitamin C

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References


Free radical scavenging is defective in tooth disease, Available at: https://www.nutraingredients.com/ Article/2002/11/26/Free-radical-scavenging-is-defective-in-tooth-disease Accessed on 07 February 2020.

Chapple ILC. Role of free radicals & antioxidants in the pathogenesis of the inflammatory periodontal diseases. J Clin Pathol 1996;49:247-55.

Chronic periodontitis, In: Newman, Takei, Klokkevold, Carranza, eds. Carranza’s Clinical Periodontology. 10th ed. New Delhi: Elsevier Publications; 2007: 494-499.

Bartold PM, Van Dyke TE. Periodontitis: A host mediated disruption of microbial homeostasis. Unlearning learned concepts. Periodontology. 2013;62:203-17.

Miller DR, Lamster IB, Chasens AI. Role of the polymorphonuclear leukocyte in periodontal health and disease. J Clin Periodontol. 1984;11:1-15.

Van Dyke TE, Levine MJ, Genco RJ. Neutrophil function and oral disease. J Oral Pathol. 1985;14:95-120.

Nguyen GT, Green ER, Mecsas J. Neutrophilsto the ROScue: Mechanisms of NADPH Oxidase Activation and Bacterial Resistance. Front Cell Infect Microbiol. 2017;7:373.

Galli C, Passeri G, Macaluso GM. FoxOs, Wnts and oxidative stress-induced bone loss: New players in the periodontitis arena? J Periodontal Res. 2011;46:397-406.

Saita M, KanekoJ, Sato T, Takahashi SS, Wada-Takahashi S, Kawamata R, et al. Novel antioxidative nanotherapeutics in a rat periodontitis model: Reactive oxygen species scavenging by redox injectable gel suppresses alveolar bone resorption. Biomaterials. 2016;76:292-301.

Wang Y, Andrukhov O, Rausch-Fan X. Oxidative Stress and Antioxidant System in Periodontitis. Front Physiol. 2017;8:910.

Sculley DV, Langley-Evans SC. Periodontal disease is associated with lower antioxidant capacity in whole saliva and evidence of increased protein oxidation. Clin Sci. 2003;105:167-72.

Panjamurthy K, Manoharan S, Ramachandran CR. Lipid peroxidation and antioxidant status in patients with periodontitis. Cell Mol Biol Lett. 2005;10:255-64.

Sugano N, Kawamoto K, Numazaki H, Murai S, Ito K. Detection of mitochondrial DNA mutations in human gingival tissues. J Oral Sci. 2000;42:221-3.

Tada A, Miura H. The Relationship between Vitamin C and Periodontal Diseases: A Systematic Review. Int J Environ Res Public Health. 2019;16:2472.

Malhotra S, Sawhney G, Pandhi P. The therapeutic potential of melatonin: a review of the science. MedGenMed. 2004;6(2):46.

Kantarci A, Thomas E, Van Dyke. Resolution of inflammation in Periodontitis. J Periodontal. 2005;76(11):2168-74.

Russel RJ, Dun-Xian, Juan C, Mayo. Melatonin as an antioxidant: Biochemical mechanisms and pathophysological implications in humans. Acta Biochemica Polonica. 2003;50(4):1129-46.

Russel RJ, Corneiro RC, Oh CS. Melatonin in relation to cellular antioxidative defence mechanisms. Horm Metab Res. 1997;29(8):263-72.

Omaye ST, Turnbull JD and Saurberlich H. Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Meth Enzymol. 1971;62:3-11.

Battino M, Bullon P, Wilson M, Newman H. Oxidative injury and inflammatory periodontal diseases. The challenge of antioxidants to free radicals and reactive oxygen species. Crit Rev Oral Biol Med. 1999;10(4):458-76.

Cutando A, Galindo P, Gomez-Moreno G. Relationship between salivary melatonin and severity of periodontal disease. J Periodontal. 2006;77:1533-8.

Nishida M, Grossi SG, Dunford RG, Ho AW, Trevisan M, Genco RJ. Dietary vitamin C and the risk for periodontal disease. J Periodontol. 2000;71:1215-23.

Chapple IL, Milward MR, Dietrich T. The prevalence of inflammatory periodontitis is negatively associated with serum antioxidant concentrations. J Nutr. 2007;137:657-64.

Kuzmanova D, Jansen ID, Schoenmaker T, Nazmi K, Teeuw WJ, Bizzarro S, et al. Vitamin C in plasma and leucocytes in relation to periodontitis. J Clin Periodontol. 2012;39(10):905-12.

Staudte H, Kranz S, Völpel A, Schütze J, Sigusch BW. Comparison of nutrient intake between patients with periodontitis and healthy subjects. Quintessence Int. 2012;43:907-16.