DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20195635

Effect of resveratrol on diabetic neuropathy in wistar albino rats

Smita Das, Jayanti Prava Behera, Y. Rojaramani, Rashmi Ranjan Mohanty

Abstract


Background: Type 2 diabetes mellitus (DM) is a common chronic disease with increasing prevalence worldwide. Prolonged uncontrolled hyperglycemia, dyslipidemia are major risk factor for its complication like neuropathy. Since there is no definite treatment for diabetic neuropathy, this study aims to evaluate the effect of resveratrol on diabetic neuropathy in high fat diet with low dose streptozotocin induced type-2 DM model in wistar albino rats.

Methods: First type 2 diabetic rat model was established. Wistar albino rats, fed with high-fat diet (HFD) rendered diabetic with streptozotocin, were divided into 6 groups, disease control (DC) treated with vehicle, standard control (SC) which received metformin, test groups treated with 5, 10, and 20 mg/kg b.w. of resveratrol and combination of half dose of metformin and resveratrol (10 mg/kg) (TC). A group of six normal animals served as normal control (NC), another six as HFD control. Fasting plasma glucose, lipid profile were measured one week after induction of diabetes. The animals were then treated orally for 2 weeks after which the same parameters were repeated. Behavioral biomarkers for neuropathy are measured in 4 weeks and 6 weeks of treatment. The in-vivo results were analyzed by one way ANOVA followed by Tukey’s multiple comparison test for biochemical parameters and Kruskal Wallis test followed by Dun’s multiple comparison test for behavioral biomarkers.

Results: Increase in fasting plasma glucose (FPG), deranged lipid profile, increased neuropathy in DC compared to NC, HFD control while a significant decrease in FBG, improved pain behavior with SC, test groups (p<0.05) as compared to the DC group.

Conclusions: Resveratrol prevents diabetic neuropathy.


Keywords


Diabetes, Resveratrol, Diabetic neuropathy

Full Text:

PDF

References


Ogurtsova K, da Rocha Fernandes JD, Huang Y, Linnenkamp U, Guariguata L, Cho NH, et al. IDF Diabetes Atlas: Global estimates for the prevalence of diabetes for 2015 and 2040. Diabetes Res Clin Pract. 2017;128:40-50.

Gill HK, Yadav SB, Ramesh V, Bhatia E. A prospective study of prevalence and association of peripheral neuropathy in Indian patients with newly diagnosed type 2 diabetes mellitus. J Postgrad Med. 2014;60:270-5.

Moxey PW, Gogalniceanu P, Hinchliffe RJ, Loftus IM, Jones KJ, Thompson MM, et al. Lower extremity amputations – a review of global variability in incidence. Diabetic Med. 2011;28:(10)1144-53.

Wilson JR, Stittsworth JD Jr, Kadir A, Fisher MA. Conduction velocity versus amplitude analysis: Evidence for demyelination in diabetic neuropathy. Muscle Nerve. 1998;21:1228-30.

Heubeck E. New Treatment Options for Diabetic Neuropathy. DOC NEWS. 2006;3(2);8-9.

Modak M, Dixit P, Londhe J, Ghaskadbi S, Paul TA. Devasagayam. Indian Herbs and Herbal Drugs Used for the Treatment of Diabetes, J Clin Biochem Nutr. 2007;40(3):163-73.

Jalili C, Roshankhah S, Moradi Y, Salahshoor MR. Resveratrol attenuates malathion-induced renal damage by declining oxidative stress in rats. Int J Pharma Investig. 2018;8:192-9.

Su HC, Hung LM, Chen JK. Resveratrol, a red wine antioxidant, possesses an insulin-like effect in streptozotocin-induced diabetic rats. Am J Physiol Endocrinol Metab. 2006;290(6):E1339-46.

Buettner R, Parhofer KG, Woenckhaus M, Wrede CE, Kunz-Schughart LA, Schölmerich J, et al. Defining high-fat-diet rat models: metabolic and molecular effects of different fat types. J Mol Endocrinol. 2006;36(3):485-501.

Rivera L, Mor´on R, Zarzuelo A, Galisteo M. Longterm resveratrol administration reduces metabolic disturbances and lowers blood pressure in obese Zucker rats. Biochem Pharmacol. 2009;77(6):105363.

Chang KJ. Effect of taurine and beta alanine on morphological changes of pancreas in streptozotocin induced diabetic rats. Adv Exp Med Biol. 2000;483:571-7.

Lu HE, Jian CH, Chen SF, Chen TM. Hypoglycaemic effects of fermented mycelium of Paecilomyces farinosus (G30801) on high-fat fed rats with streptozotocin-induced diabetes, Indian J Med Res. 2010;131:696-701.

Friedewald WT, Levy RI, Frederickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. Clin Chem. 1972;18:499-502.

Kawakita K, Funakoshi M. A quantitative study on the tail flick test in rat. Physiol Behav. 1987;39(2):235-40.

Bardin L, Malfetes N, Newman-Tancredi A, Depoortre R. Chronic restraint stress induces mechanical and cold allodynia and enhances inflammatory pain in rat: Relevance to human stress associated painful pathologies. Behavioural Brain Res. 2009;205:360-6.

Abbott FV, Franklin KBJ, Westbrook RF. The formalin test: scoring properties of the first and second phases of the pain responses in rats. Pain. 1995;60:91-102.

Bijender S, Harish D, Rishi S, Patil BM. Effect of vitamin E on the impaired gastrointestinal activity of streptozotocin induced diabetic rats. IJP. 2003;35:186-7.

Rodrigues B, Kong MCCJ, Goyal RK, McNeil JH, Strain differences in susceptibility to streptozotocin-induced diabetes: effects on hypertriglyceridemia and cardiomyopathy. Cardiovascular Res. 1997;34(1):199-205.

Szkudelski T. The mechanism of alloxan or streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001;50(6):537-46.

Jung JY, Lim Y, Moon MS, Kim JY, Kwon O. Onion peel extracts ameliorate hyperglycemia and insulin resistance in high fat diet/streptozotocin induced diabetic rats. Nutr Metab (Lond). 2011;8:18.

Kumar NP, Annamalai AR, Thakur RS. Antinociceptive property of Emblica officinalis Gaertn (Amla) in high fat diet-fed/low dose streptozotocin induced diabetic neuropathy in rats. Indian J Exp Biol. 2009;47(9):737-42.

Pradeepa R, Rema M, Vignesh J, Deepa M, Deepa R, Mohan V. Prevalence and risk factors for diabetic neuropathy in an urban south Indian population: The Chennai Urban Rural Epidemiology Study (CURES55). Diabet Med. 2008;25:407-12.

U.K. Prospective Diabetes Study Group. Eeffect of intensive blood glucose-control with metformin on complications in overweight patients with type 2 diabetes (UKPDS). Lancet. 1998;352:854-65.

Shahi MM, Haidari F, Shiri MR. Comparison of effect of resveratrol and vanadium on diabetes related dyslipidemia and hyperglycemia in streptozotocin induced diabetic rats. Adv Pharma Bulletin. 2011;1(2):81-6.

Freshwater JD, Svensson CI, Malmberg AB, Calcutt NA. Elevated spinal cyclooxygenase and prostaglandin release during hyperalgesia in diabetic rats. Diabetes. 2002;51(7):2249-55.

Sharma S, Kulkarni SK, Chopra K. Effect of resveratrol, a polyphenolic phytoalexin, on thermal hyperalgesia in a mouse model of diabetic neuropathic pain. Fundam Clin Pharmacol. 2007;21(1):89-94.

Kumar A, Kaundal RK, Iyer S, Sharma SS. Effects of resveratrol on nerve functions, oxidative stress and DNA fragmentation in experimental diabetic neuropathy. Life Sci. 2007;80(13):1236-44.

Koch KL, Calles-Escandón J. Diabetic gastroparesis. Gastroenterol Clin North Am. 2015;44(1):39-57.