DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20195290

Evaluation of alloxan on induction of diabetes in albino rats

Soni ., A. N. Mishra

Abstract


Background: Alloxan-induced diabetes model is used as a “study tool” to elucidate the pathophysiology of the disease and much more as a “search engine” for antidiabetic compounds with better therapeutic characteristics. It was the first agent used in the category of chemically induced diabetes to create a model of insulin dependent diabetes mellitus. Other chemicals being streptozocin, dexamethasone, insulin antibodies-induced diabetes.

Methods: Albino rats were divided into four groups with ten rats in each group. Alloxan monohydrate 2%, solution which was dissolved in 0.9% of sodium chloride (normal saline) as a diluent and given intraperitoneally to rats and blood glucose estimation made by using glucometer. Total 40 albino rats were taken and divided into 4 groups. 10 rats receiving normal saline were grouped as Group A, 10 rats received alloxan at a dose of 150 mg/kg as Group B, 10 rats received alloxan at a dose of 160 mg/kg as Group C and 10 rats received alloxan at a dose of 170 mg/kg as Group D.

Results: Highest rate of mortality and alopecia were noted in group D receiving alloxan at a dose of 170 mg/kg whereas highest percentage of fluctuation in fasting blood glucose range was seen in group C receiving alloxan at a dose of 160 mg/kg.

Conclusions: Such unpredictable response shows that alloxan is not ideal drug for induction of diabetes in experimental animal. Mortality, fasting blood glucose returning to non-diabetic range and alopecia are the chief drawbacks.


Keywords


Alloxan, Alopecia, Diabetes

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References


Lumelsky N, Blondel O, Laeng P, Velasco I, Ravin R, McKay R. Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets. Science. 2001;292(5520):1389-94.

Muoio DM, Newgard CB. Molecular and metabolic mechanisms of insulin resistance and β-cell failure in type 2 diabetes. Nature Reviews Molecul Cell Biol. 2008;9(3):193.

Busnardo AC, DiDio LJ, Tidrick RT, Thomford NR. History of the pancreas. Am J Surg. 1983;146(5):539-50.

Rees DA, Alcolado JC. Animal models of diabetes mellitus. Diabetic Med. 2005;22(4):359-70.

Liebig LJ, Wohler F. A History of Chemistry. Palgrave, London: 1964: 294-336.

Black HE, Rosenblum IY, Capen CC. Chemically induced (streptozotocin-alloxan) diabetes mellitus in the dog: biochemical and ultrastructural studies. Am J Pathol. 1980;98(2):295.

Ahmed SK, Sunil M, Cheekavolu C, Alasyam N. Evaluation of antidiabetic effect of Murrayakoenigii leaves chloroform extract (MKLCE) in alloxan induced diabetic albino rats. 20187;11(6):474-7.

Care V. CPCSEA guidelines for laboratory animal facility. Indian J Pharmacol. 2003;35:257-74.

Ighodaro MO, Adeosun MA, Akinloye AO. Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Medicina. 2017;53(6):365-74.

Gerich JE, Charles MA, Grodsky GM. Regulation of pancreatic insulin and glucagon secretion. Annual Review Physiol. 1976;38(1):353-88.

Szkudelski T. Streptozotocin–nicotinamide-induced diabetes in the rat. Characteristics of the experimental model. Exp BiolMed. 2012;237(5):481-90.