DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20185157

Comparative study of anti-inflammatory property of calcium channel blocker and aspirin in albino rats

Anjum Jabeen

Abstract


Background: Calcium channel blockers are being used in the treatment of hypertension, ischemic heart disease, cardiac arrhythmias etc. They act by blocking the slow calcium channels. Influx of calcium is an essential step in the release of histamine and 5HT from mast cells and in the synthesis and release of prostaglandins. They are the main mediators in the process of inflammation. The aim of the present study was to compare anti-inflammatory properties of calcium channel blockers and aspirin in albino rats.

Methods: Present study was prospective intervention study carried out to compare anti-inflammatory properties of calcium channel blockers and aspirin in albino rats. Total 30 albino rats were used, and they were divided into 5 groups of 6 each. First group of rats was control group where normal saline was used. Second group was standard group where aspirin was used. Remaining three groups were test groups and given nifedipine, amlodipine and diltiazem respectively. Effects were observed and compared between the groups.

Results: In carrageenan method, the anti-inflammatory response of Aspirin was significantly higher. Nifedipine response was <aspirin but >diltiazem. The response of amlodipine was not significantly > that of control percent inhibition. In histamine method, the anti-inflammatory response of aspirin was significantly highest. Anti-inflammatory response of nifedipine was < aspirin but > diltiazem. Diltiazem response was < nifedipine but > amlodipine. In formaldehyde method, aspirin inhibition was highest at 96.2% followed by nifedipine (90.7%), diltiazem (75.9%) and amlodipine (3.7%). In cotton wool pellet granuloma, the mean dry granuloma weight was least for aspirin and percent anti-inflammatory activity was significantly high.

Conclusions: Calcium channel blockers (nifedipine, diltiazem) have shown comparable anti-inflammatory property with that of aspirin. Further clinical studies are required for confirmation.


Keywords


Albino rats, Amlodipine, Diltiazem, Effects, Nifedipine

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References


Paterson JR, Baxter G, Dreyer JS, Halket JM, Flynn R, Lawrence JR. Salicylic acid sans aspirin in animals and man: persistence in fasting and biosynthesis from benzoic acid. J Agricultural Food Chem. 2008 Nov 20;56(24):11648-52.

Lewis HD, Davis JW, Archibald DG, Steinke WE, Smitherman TC, Doherty JE, et al. Protective effects of aspirin against acute myocardial infarction and death in men with unstable angina. N Engl J Med. 1983 Aug 18;309(7):396-403.

Julian DG, Chamberlain DA, Pocock SJ. A comparison of aspirin and anticoagulation following thrombolysis for myocardial infarction (the AFTER study): a multicentre unblinded randomised clinical trial. BMJ. 1996 Dec 7;313(7070):1429-31.

Krumholz HM, Radford MJ, Ellerbeck EF, Hennen J, Meehan TP, Petrillo M, et al. Aspirin in the treatment of acute myocardial infarction in elderly Medicare beneficiaries: patterns of use and outcomes. Circulation. 1995 Nov 15;92(10):2841-7.

Algra AM, Rothwell PM. Effects of regular aspirin on long-term cancer incidence and metastasis: a systematic comparison of evidence from observational studies versus randomised trials. Lancet Oncol. 2012 May 1;13(5):518-27.

Rothwell PM, Price JF, Fowkes FG, Zanchetti A, Roncaglioni MC, Tognoni G, et al. Short-term effects of daily aspirin on cancer incidence, mortality, and non-vascular death: analysis of the time course of risks and benefits in 51 randomised controlled trials. Lancet. 2012 Apr 28;379(9826):1602-12.

Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for antiinflammatory drugs. Proceed Soc Exp Biol Med. 1962 Dec;111(3):544-7.

Maurya R, Yadav DK, Singh G, Bhargavan B, Murthy PN, Sahai M, et al. Osteogenic activity of constituents from Butea monosperma. Bioorganic Med Chem Lett. 2009 Feb 1;19(3):610-3.

Lake-Bakaar G, Lyubsky S. Dose-dependent effect of continuous subcutaneous verapamil infusion on experimental acute pancreatitis in mice. Digestive Dis Sci. 1995 Nov 1;40(11):2349-55.

Rödler S, Roth M, Nauck M, Tamm M, Block LH. Ca2+-channel blockers modulate the expression of interleukin-6 and interleukin-8 genes in human vascular smooth muscle cells. J Mol Cellular Cardiol. 1995 Oct 1;27(10):2295-302.

Southan GJ, Szabó C. Selective pharmacological inhibition of distinct nitric oxide synthase isoforms. Biochem pharmacol. 1996 Feb 23;51(4):383-94.

Craven PA, DeRubertis FR. Ca2+. Calmodulin-dependent release of arachidonic acid for renal medullary prostaglandin synthesis. Evidence for involvement of phospholipases A2 and C. J Biol Chem. 1983 Apr 25;258(8):4814-23.

Aditya GN, Chattopadhyay RN, Mandal S, Roy RK, Lahiri HL, Maitra SK. Preliminary study on anti-inflammatory effect of calcium channel blockers in albino rats. Indian J Pharmacol. 1997 Mar 1;29(2):132.

Khaksari M, Mahani SE, Mahmoodi M. Calcium channel blockers reduce inflammatory edema in the rat: Involvement of the hypothalamus-pituitary-adrenal axis. Indian J Pharmacol. 2004 Nov 1;36(6):351-4.

Gitanjali K, Gupta B, Vijay T. Anti-inflammatory activity of calcium channel blockers. Int J Life Sci Pharma Res. 2012;2(2):1-6.