Published: 2017-09-23

To evaluate the anticonvulsant activity of ethanolic extract of Moringa oleifera (drumstick leaves) in albino mice

Sushma V. Naidu, Harsha R., Jyothsnya S.


Background: To evaluate the anti-convulsant activity of ethanolic extract of Moringa oleifera (Drum stick leaves) in seizure induced albino mice and to compare it with standard drug Sodium valproate.

Methods: Swiss albino mice of either sex weighing around 25-30g were randomly selected and divided into four groups of six mice each. Group 1: control- treated with gum acacia. Group 2: Standard - Valproic acid 40mg/kg body weight. Group 3: T1- ethanolic extract of Moringa oleifera (150mg/kg). Group 4: T2 - ethanolic extract of Moringa oleifera (300mg/kg). All drugs were administered orally one hour prior to induction of seizure. The anticonvulsant activity was screened using maximal electroshock seizure (MES) model and pentylenetetrazole (PTZ) model.

Results: Results were analysed by ANOVA followed by Bonferroni’s post hoc test. Abolition of Tonic hind limb extension was taken as the protective end point against MES induced seizures and prolongation of seizure latency in PTZ model.

At both the doses the ethanolic extract of Moringa oleifera significantly (p value <0.05) reduced the duration of hind limb extension in MES test and also significantly (p value <0.05) delayed the onset of clonic seizures in PTZ induced convulsion when compared with control group.

Conclusions: On comparing the percentage protection offered by Moringa oleifera leaves against both MES and PTZ model, it possesses significant anticonvulsant activity at both doses, with more efficacy at 300mg/kg BW indicating that the test drug can prove a very promising drug for treatment of epilepsy. Further studies are required for isolation and identification of the active constituent.


Anti-convulsant, Moringa oleifera, MES, PTZ

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Sridharan R, Murthy BN. Prevalence and pattern of epilepsy in India. Epilepsia. 1999;40(5):631-6.

Kamboj P, Singh I, Mahadevan N, Chaudhary G. Anticonvulsant from nature. Phcog Rev. 2009;108-17. Available at:

Roy MK, Das D. Indian Guidelines on epilepsy. IAP expert committee guidelines. 528-32.

Jayaram R, Anitha T, Joshi VD. Analgesic and anticonvulsant effects of Acoruscalamus roots in mice. International Journal of Pharma Tech Research. 2010;2(1):552-5.

Kesharwani S, Prasad P, Roy A, Sahu RK. An overview on Phytochemistry and Pharmacological Exploration of Moringa Oleifera. UK Journal of Pharmaceutical and Biosciences. 2014;2(1):34-41.

Farooq A, Latif S, Muhammad A, Gilani AH. Moringaoleifera: A Food Plant with Multiple Medicinal Uses. Phytotherapy Research. 2007;(21):17-25.

Goyal BR, Agrawal BB, Goyal RK, Mehta AA. Phytopharmacology of Moringaoleifera lam an overview. Natural Product Radiance. 2007;6(4):347-53.

Gantara TH, Joshi UH, Bhalodia PN, Desai TR, Tirgar PR. A panoramic view of pharmacognostic, pharmacological, nutritional, therapeutic and prophylactic values of Moringaoleifera Lam. International Research Journal of Pharmacy. 2012;3(6):1-7.

Mc Namara JO, Hardman JG, Limbird JE, Molioff PB, Ruddon RW, Gillman AG. Drugs effective in the treatment of epilepsies. Goodman and Gillman s The Pharmacological basis of Therapeutics.12th edition. NewYork: Mc Graw Hill; 2012:583-586.

Muhammad R, Abdullah A, Alghasham, Mohammad SA. Potentation of valporate induced anticonvulsant response by Nigella sativaseed constituents -The role of GABA receptor. International Journal of Health sciences. 2008;2(1):15-25.

Raza M, Saheen F, Choudhary MI, Sombati S, Rafiq A, Suria A, et al. Anticonvulsant activities of ethanolic extract and aqueous fraction isolated from delphinium denudatum. J Ethnopharmacol. 2001;78(1):73-8.

De Sarro A, Cecchetti V, Frarolini V, Naccari F, Tabarrini O, De Sarro G. Effect of novel 6-desfluoroquinolones and classic quinolones on pentylenetetrazole induced seizure in mice. Antimicrob agents. Chemother. 1999;43:1729-36.

Fernandez SP, Wasowski C, Loscalzo LM, Granger RE, Gohnston GA, Paladini AC, et al. Central nervous system depressant action of flavonoid glycosides. Eur J Pharmacol. 2006;539:168-76.

Krall RL, Penry JK, White BG, Kuferberg HJ, Swinyar Eug A. Antiepileptic drug development II. Anticonvulsant Drug screening. Epilepsia. 1978;19(4):409-28.

Porter RJ, Cereghino JJ, Gladding GD, Hessie BJ, Kupferberg HJ, Scoville B, et al. Antiepiletic Drug Development program. Cleve clinQ. 1984;51(2):293-305.