DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20174378

Study of the oral hypoglycemic activity of Moringaoleifera leaves alone and in combination with Glibenclamide in streptozotocin induced diabetic albino rats

Sushma V. Naidu, Suresha R. N., Jayanthi M. K., Satish A. M., Kalabharathi H. L., Pushpa V. H.

Abstract


Background: Oringaoleifera is a widely used plant with high medicinal value, well known for its pharmacological actions and is used in various conditions. It has been reported to have many biological properties like anti-inflammatory, antimicrobial, antispasmodic, antitumour including antidiabetic activity.

Methods: The study was carried out in Wistar albino rats with body weight 150-250gms. Diabetes was induced by injecting Streptozotocin intraperitoneally- dose 55 mg/kg BW. Animals were divided into 5 groups with 6 animals in each group. First group (Control) was given 2% gum acacia. Other 4 groups were induced diabetes by giving Streptozotocin. Diabetic control group received gum acacia (0.5 ml), Standard group received Glibenclamide (0.5mg/kg BW), Test group received Moringaoleifera extract (300mg/kg) and Test+ Standard group receiving combination of Moringaoleifera and glibenclamide at half the above doses. All drugs were given orally for 28 days and blood glucose levels analyzed using Glucometer on Day 0 before drug and on D1, D3, D7, D14, D21, and D28. Data were statistically analyzed by ANOVA and Tukey‘s Post Hoc test.

Results: Hypoglycemia produced by Moringaoleifera extract was significant (p<0.001) when compared to diabetic control group from day 7 to day 28. The percent reduction of blood glucose level was 52.9% as compared to Glibenclamide group 61.3%. The combination group also showed significant hypoglycemic activity the percentage reduction being 56.44%.

Conclusions: Thus, Moringaoleifera decreased blood glucose level efficaciously as compared to diabetic control group and similar to standard group at p<0.001.


Keywords


Diabetes mellitus, Hypoglycemia, Moringaoleifera, Streptozotocin

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References


Alvil CP. Diabetes mellitus in. Kasper Braunwald, Fauci, Hauser, Longo, Jameson. Harrison’s principles of internal medicine.17th Ed. New York: Mcgraw Hill; 2008:2152.

King H, Rewers M. Diabetes in adults is now a Third World problem. The WHO Ad Hoc Diabetes reporting Group. Bull World Health Organ. 1991;69:643-8.

Anjana RM, Ali MK, Pradeepa R, Deepa M, Dutta M, Unnikrishnan R, et al. The need for obtaining accurate Nationwide estimates of Diabetes Prevalence in India- Rationale for national study on Diabetes. Indian J Med Res. 2011;133:369-80.

Paul Z, Shaw J. Diabetes- A World wide Problem, Joslin Diabetes mellitus. Lippincott Williams & Wilkins. A Wolters Kluwer Company; 2006:525.

Ganguly R, Guha D. Alteration of brain monoamines & EEG wave pattern in rat model of Alzheimer’s disease & protection by Moringaoleifera. Indian J Med Res. Dec 2008;128:744-51.

Chinmoy K. Bose. Possible Role of Moringaoleifera Lam. Root in Epithelial Ovarian Cancer. Medgenmed. 2007;9(1):26.

Jaiswal D, Rai PK, Kumar A, Mehta S, Watal G. Effect of Moringaoleifera Lam. Leaves aqueous extract therapy on hyperglycemic rats. Journal Ethnopharmacology. 2009;123:392-6.

Moringaoleifera for Diabetes - Published article by Avon Howard, accessed on 14.07.2013. Available at: Http://www.upublish.info/Article/Moringa-Oleifera-for-Diabetes/313162.

Iffiú-Soltész Z, Wanecq E, Lomba A, Portillo MP, Pellati F, Szöko E, et al. Chronic benzylamine administration in the drinking water improves glucose tolerance, reduces body weight gain and circulating cholesterol in high-fat diet-fed mice. Pharmacol Res. 2010 Apr;61(4):355-63.

Arumugam KR, Murugesh N. Text Book of Pharamacognosy. 2nd Edition. 1990:33-35.

Motyl K, McCabe LR. Streptozotocin, Type I Diabetes Severity and Bone. Biological Procedures Online. 2009;11:296-315.

Satish Sekar D, Subramanian S. Antioxidant properties of Momordicacharantia (Bitter gourd) seeds on streptozotocin induced diabetic rats. Asia Pac J Clin Nutr. 2005;14(2):153-8.

Saha P, Thamil SV, Mondal SK, Mazumder UK, Gupta M. Antidiabetic and antioxidant activity ofmethanol extract of ipomoea reptanspoir aerial parts in streptozotocin induced diabetic rats. Pharmacologyonline. 2008;1:409-21.

Jae-Jeong L, Ho-Youg Y, Jae-Won Y, Jun-Seop S, Jai-Hyun K, Chan Wha K. Characterization of STZ-induced Diabetic Rats and Pharmacodynamics of Insulin Formulations. Biosci. Biotechnil. Biochem. 2003;67(11):2396-401.

Tende JA, Ezekiel I, Dikko AU, Goji DT. Effect of Ethanolic Leaves Extract of Moringaoleiferaon Blood Glucose Levels of Streptozocin-Induced Diabetics and Normoglycemic Wistar Rats. British Journal of Pharmacology and Toxicology. 2011;2(1):1-4.

Adisakwattana S, Chanathong B. Alpha-glucosidase inhibitory activity and lipid-lowering mechanisms of Moringaoleifera leaf extract. Eur Rev Med Pharmacol Sci. 2011 Jul;15(7):803-8.

Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine. 1995;2:137-87.