The role of antioxidants and free radicals in the healing effects of Bacopa monniera on acetic acid-induced colitis in rats
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20174365Keywords:
Antioxidants, Bacopa monniera, Colitis, Free radicalsAbstract
Background: The aim to study and elucidate the healing effects of ethanolic extract of dried whole plant of Bacopa monniera against experimental colitis in rats.
Methods: Bacopa monniera whole plant extract was administered orally, once daily for 14 days, to rats after induction of colitis with acetic acid. We studied its effects on: faecal output, food and water intake, and body weight changes and also examined colonic mucosal damage, inflammation and status of antioxidants: superoxide dismutase, reduced glutathione; free radicals: nitric oxide, lipid peroxidation on 15th day of the experiment. Antibacterial activity of the extract was also studied using in vitro procedures. Statistical comparison was performed using either unpaired ‘t’ test or one -way analysis of variance (ANOVA) and for multiple comparisons versus control group was done by Dunnett’s test.
Results: Bacopa monniera whole plant extract decreased colonic mucosal damage, inflammation, faecal output and increased body weight in acetic acid induced colitis. It also showed antibacterial activity and enhanced the antioxidant but decreased free radicals. Acute toxicity study indicated no mortality or other ANS or CNS related adverse effects even with ten time effective dose indicating its safety.
Conclusions: Bacopa monniera whole plant extract is safe, effective and could be beneficial as a complementary agent in treatment of ulcerative colitis.
References
Udupa KN, Singh RH. Clinical and Experimental Studies on Rasayana drugs and Panchkarma Therapy, Central Council for Research in Ayurveda and Siddha. New Delhi; 1995.
Shabana CA, Muhammad DSA, Atta-ur-Rahman Y. Anti-inflammatory activity of Bacopa monniera in rodents. J Ethnopharmacol. 2006;104:286-9.
Kishore K, Singh M. Effect of bacosides, alcoholic extract of Bacopa monniera Linn. (Brahmi), on experimental amnesia in mice. Indian J Exp Biol. 2005;43:640-5.
Russo A, Angelo AI, Francesca B, Marcella R, Angelo V. Free radical scavenging capacity and protective effect of Bacopa monniera L. on DNA damage. Phytother Res. 2003;17:870-5.
Garai S, Mahato SB, Ohtani K, Yamasaki K. Dammarane- type Triterpenoid saponins from B. monniera. Phytochemistry. 1996;42:815-20.
Sairam K, Dorababu M, Goel RK, Bhattacharya SK. Antidepressant activity of standardized extract of Bacopa monniera in experimental models of depression in rats. Phytomedicine. 2002;9:207-11.
Sairam K, Rao Ch V, Dora Babu M, Goel RK. Prophylactic and curative effects of Bacopa monniera in gastric ulcer models. Phytomedicine 2001;8:423-30.
Bhattacharya SK, Bhattacharya A, Kumar A, Ghosal S. Antioxidant activity of B. monniera in rat frontal cortex, striatum, and hippocampus. Phytotherapy Research. 2000;14:174-79.
Vijayan V, Helen A. Protective activity of Bacopa monniera Linn on nicotine induced toxicity in mice. Phytotherapy Research. 2007;21:378-81.
Hanauer SB. Inflammatory bowel disease: epidemiology. Pathogenesis and Therapeutic Opportunities. 2006;12:S3-9.
Hanauer S, Sninsky CA, Robinson M. An oral preparation of mesalamine as long-term maintenance therapy for ulcerative colitis. Ann Intern Med. 1996;124:204-11.
Keshavarzian A, Morgan G, Sedghi S, Gordon JH, Doria M. Role of reactive oxygen metabolites in experimental colitis. Gut. 1990;31:786-90.
Grisham MB, Granger DN. Neutrophil-mediated mucosal injury. Role of reactive oxygen metabolites. Dig Dis Sci. 1988;33:6S-15S.
Rahimi R, Mozaffari S, Abdollahi M. On the Use of Herbal Medicines in Management of Inflammatory Bowel Diseases: A Systematic Review of Animal and Human Studies. Dig Dis Sci. 2009;54:471-80.
Lowry OH, Rosenborough NJ, Farr AL, Randal RJ. Protein measurement with folin phenol reagent. J Biol Chem. 1951;193:265-75.
Okhawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:351-8.
Miranda KM, Epsey MG, Wink DA. A rapid, simple spectophotometric method for simultaneous detection of nitrate and nitrite. Biol Chem. 2001;5:62-71.
Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase, Indian J Biochem Biophy. 1984;21:130.
Sedlak J, Lindsay RH. Estimation of total protein bound and nonprotien sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem. 1968;25:192.
Singh RK, Nath G. Antimicrobial activity of Elaeocarpus sphaericus. Phytother Res. 1999;13:448-50.
Irith W, Kai H, Robert EW H. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols. 2008;3:163-75.
Asakura H. Ulcerative colitis and intestinal bleeding. Nippon Rinsho. 1998;56:2354-9.
Shanahan F, Bernstein CN. The evolving epidemiology of inflammatory bowel disease. Current Opinion in Gastroenterology. 2009;25:301-5.
Channa S, Dar A, Anjum S, Yaqoob M, Rahman A. Anti-inflammatory activity of Bacopa monniera in rodents. Journal of Ethnopharmacology. 2006;104:286-9.
Pfeiffer CJ, Qiu BS. Effects of chronic nitric oxide synthase inhibition on TNB-induced colitis in rats. J. Pharm. Pharmacol. 1995;47:827-32.
