Antiangiogenic activity of zinc and zinc-sorafenib combination using the chick chorioallantoic membrane assay: a descriptive study

Authors

  • Manu Kumar Department of Pharmacology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India
  • Girish Gulab Meshram Department of Pharmacology, Postgraduate Institute of Medical Education and Research and Dr. Ram Manohar Lohia Hospital, New Delhi, India
  • Tripti Rastogi Department of Pharmacology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India
  • Sonal Sharma Department of Pathology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India
  • Rachna Gupta Department of Pharmacology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India
  • Seema Jain Department of Pharmacology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India
  • Anisha Prasad Department of Pharmacology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India
  • Vikas Galav Central Animal House, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India
  • S. K. Bhattacharya Department of Pharmacology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20171559

Keywords:

Aantiangiogenic, Chick chorioallantoic membrane assay, Sorafenib, Zinc

Abstract

Background: Zinc, a trace element, is known for downregulating several proangiogenic growth factors and cytokines. However, its antiangiogenic activity is not adequately studied. The present study was aimed to evaluate the possible antiangiogenic activity of zinc via the chick chorioallantoic membrane (CAM) assay. Also, the antiangiogenic activity of the combination therapy of zinc with various doses of sorafenib, a tyrosine kinase inhibitor, was evaluated.

Methods: A pilot study was initially conducted so as to select suitable doses of zinc and sorafenib. The antiangiogenic activity after combining zinc 2.5 μg/embryo with sorafenib 1, and 2 μg/embryo was also evaluated. The antiangiogenic activity was quantified in terms of total length of blood vessels, number of junctions, number of branching points, and mean length of the blood vessels.

Results: Zinc 2.5 μg/embryo showed significant (p <0.05) antiangiogenic activity, as compared to the control group. However, its effect was not comparable to that of sorafenib 2 μg/embryo. The combination of zinc 2.5 μg/embryo with sorafenib 2 μg/embryo did not show an additive/synergistic effect. The combination of zinc 2.5 μg/embryo with sorafenib 1 μg/embryo produced an antiangiogenic activity which was comparable (p >0.05) to that of sorafenib 2 μg/embryo.

Conclusions: Zinc caused significant antiangiogenic activity in the CAM assay. The lack of addition/synergism in the zinc-sorafenib combination could have been due to the variability in the dose/ratio selection. Addition of zinc to sorafenib therapy could improve treatment tolerability, reduce cost of therapy, and reduce the emergence of drug resistance. Future mechanistic studies could identify the exact pharmacodynamics of zinc as an angiogenesis inhibitor.

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Published

2017-04-24

How to Cite

Kumar, M., Meshram, G. G., Rastogi, T., Sharma, S., Gupta, R., Jain, S., Prasad, A., Galav, V., & Bhattacharya, S. K. (2017). Antiangiogenic activity of zinc and zinc-sorafenib combination using the chick chorioallantoic membrane assay: a descriptive study. International Journal of Basic & Clinical Pharmacology, 6(5), 1060–1065. https://doi.org/10.18203/2319-2003.ijbcp20171559

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Original Research Articles