Evaluation of anti-depressant effect of lemon grass (Cymbopogon citratus) in albino mice

Sujata Dudhgaonkar, Manali Mahajan, Swapnil Deshmukh, Pallavi Admane, Huma Khan

Abstract


Background: Depression is a common serious psychiatric disorder and the available anti-depressant treatments are associated with many unwanted side-effects. Thus, various herbal products have been tried. The advantages of herbal treatments would include its complementary nature to the conventional treatment, thus making the latter a safer and cheaper option for depressive disorders. The objective of the present study was to evaluate the anti-depressant activity of lemon grass (Cymbopogon citratus) in albino mice and compare it with Imipramine.

Methods: A total of 60 Swiss albino mice weighing around 20-40 g of either sex were divided into 10 groups (n=6). They were orally administered with tween 80, as a control, 20 mg/kg imipramine (standard), 5 mg/kg and 10 mg/kg C. citratus (test drugs), and combination of imipramine (10 mg/kg) and C. citratus (10 mg/kg).  Duration of immobility was observed for last 4 mins of total 6 mins period in groups 1-5 for forced swimming test (porsolt test) and groups 6-10 for tail suspension test each on 1st, 8th and 15th day and recorded as mean±standard error of the mean. Results were analyzed by one-way analysis of variance, followed by Tukey’s post-hoc test.

Results: Lemon grass at the above doses significantly reduced the immobility time in both the tests compared with the control (<0.05). The reduction in the duration of immobility at the dose of 10 mg/kg was comparable to imipramine.

Conclusions: The essential oil of lemon grass (C. citratus) has significant anti-depressant activity comparable to imipramine.


Keywords


Anti-depressant, Imipramine, Cymbopogon citratus

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References


O’Donnell MJ, Shelton CR. Drug therapy of depression and anxiety disorders. Goodman and Gilman’s. The Pharmacological Basis of Therapeutics. 12th Edition, Chapter 15. New York: McGraw Hill; 2011: 397.

Gupta SK. Antidepressant agents. Drug Screening Methods (Preclinical Evaluation of New Drugs). 2nd Edition. New Delhi: Jaypee; 2009: 27, 392.

Fauci AS, Braunwald E, Kasper D, Hauser S, Longo D, Jameson J, et al., editors. Harrison’s Principles of Internal Medicine. 17th Edition. New York: McGraw Hill Medical; 2008: 2717-8.

Nyarko DH, Barku YV. Batama J. Antimicrobial examinations of Cymbopogon citratus and adiatum capillus veneris used in ghanaian folkoric medicine. Int J Life Sci Pharm Res 2012;2(2):L115-8.

American Psychiatric Association. DSM-IV TR. Washington, D.C. APA; 2000 with permission as reproduced in Sadock BJ, Sadock VA, editors. Synopsis of Psychiatry. 9th Edition. New York: Lippincott Williams and Wilkins; 2003: 542.

Solomon DA, Keller MB, Leon AC, Mueller TI, Lavori PW, Shea MT, et al. Multiple recurrences of major depressive disorder. Am J Psychiatry. 2000;157(2):229-33.

Davies KJ. Oxidative stress: the paradox of aerobic life. Biochem Soc Symp.1995;61:1-31.

Filomeni G, Ciriolo MR. Redox control of apoptosis: an update. Antioxid Redox Signal. 2006;8(11-12):2187-92.

Davies KJ. Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. IUBMB Life. 2000;50(4-5):279-89.

Sies H. Oxidative stress: oxidants and antioxidants. Exp Physiol. 1997;82(2):291-5.

Halliwell B. Oxidative stress and neurodegeneration: where are we now? J Neurochem. 2006;97(6):1634-58.

Porsolt RD. Animal models of depression: utility for transgenic research. Rev Neurosci. 2000;11(1):53-8.

McLeod TM, López-Figueroa AL, López-Figueroa MO. Nitric oxide, stress, and depression. Psychopharmacol Bull. 2001;35(1):24-41.