A modified method for evaluating analgesic activity of drugs using Rana tigrina frog

Swanand Pathak, Prabhakar R. Patil, Harshata Suresh Harti, Rohit Dixit

Abstract


Background: Different species of frogs had been used by many researchers for evaluation of analgesic drugs e.g., Rana pipiens and African claw frog. In our study, we used Rana tigrina, which was never used for evaluation of analgesic activity of drugs. So by doing this project, we judged usefulness of R. tigrina to evaluate analgesic drugs.

Methods: Animals used were R. tigrina of either sex weighing 100-150 g. Glass flask with porous platform was used for observation of frog. All groups were treated with 4% NaCl solution S.C. on abdomen). Characteristic parameter i.e., number of eye blinkings (this parameter was observed during the pilot study after 4% NaCl S.C. injection on lower third of frogs abdominal wall) were observed before and after drug administration. Each observation was for 5 mins. Centrally and periphrally acting drugs effect was tested on the number of blinks and buccal oscillations.

Results: Centrally acting drug inhibit rise in number of eye blinkings and buccal oscillation significantly. Piroxicam diclofenac sodium and ketoprofen decreased rise in number of blinks; but, it was not significant as compared with control.

Conclusion: This animal may be used alternative to existing methods of evaluating analgesics in Indian setup.


Keywords


Rana tigrina, Writhing, Analgesic activity, Animal model

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References


Gutstein HB, Akil H. Opioid analgesics. In: Goodman & Gilman’s the Pharmacological Basis of Therapeutics. 11th Edition. New York, NY: McGraw-Hill; 2005: 583-4.

Vogel HG, Vogel WH, Scholkens BA, Sandow J, Muller G, Wolfgang F. Analgesic, anti-inflammatory, and anti-pyretic activity. Vogel Drug Discovery and Evaluation Pharmacological Assay. 2nd Edition. Berlin, Heidelberg: Springer-Verlag; 2002: 670.

Chakravarthi VP, Gopalkumar N. Evaluation of analgesic activity of lotus seeds (Nelumbo nucifera) in Albino rats. Vet World. 2009;2(9):355-7. Available from: http://www.veterinaryworld.org.

Heidari MR, Mehrabani M, Pardakhty A, Khazaeli P, Zahedi MJ, Yakhchali M, et al. The analgesic effect of Tribulus terrestris extract and comparison of gastric ulcerogenicity of the extract with indomethacine in animal experiments. Ann N Y Acad Sci. 2007;1095:418-27.

Clark JS, Bentley E, Smith LJ. Evaluation of topical nalbuphine or oral tramadol as analgesics for corneal pain in dogs: a pilot study. Vet Ophthalmol. 2011;14(6):358-64.

Manning BH, Merin NM, Meng ID, Amaral DG. Reduction in opioid-and cannabinoid-induced antinociception in rhesus monkeys after bilateral lesions of the amygdaloid complex. J Neurosci. 2001;21(20):8238-46.

Stevens CW, Rothe KS. Supraspinal administration of opioids with selectivity for mu-delta- and kappa-opioid receptors produces analgesia in amphibians. Eur J Pharmacol. 1997;331(1):15-21.

Stevens CW, MacIver DN, Newman LC. Testing and comparison of non-opioid analgesics in amphibians. Contemp Top Lab Anim Sci. 2001;40(4):23-7.

Coble DJ, Taylor DK, Mook DM. Analgesic effects of meloxicam, morphine sulfate, flunixin meglumine, and xylazine hydrochloride in African-clawed frogs (Xenopus laevis). J Am Assoc Lab Anim Sci. 2011;50(3):355-60.

ANZCCART. The University of Adelaide Australia. Available from: http://www.adelaide.edu.au/ANZCCART/publications/A13_FrogsandToadsFactSheet.pdf.[Last accessed on 2013 Sep 1].

Fukawa K, Kawano O, Hibi M, Misaki N, Ohba S, Hatanaka Y. A method for evaluating analgesic agents in rats. J Pharmacol Methods. 1980;4(3):251-9.

Pezalla PD, Stevens CW. Behavioral effects of morphine, levorphanol, dextrorphan and naloxone in the frog Rana pipiens. Pharmacol Biochem Behav. 1984;21(2):213-7.

Suckow MA, Terril LA, Grigdesby CF, March PA. Evaluation of hypothermia-induced analgesia and influence of opioid antagonists in leopard frogs (Rana pipiens). Pharmacol Biochem Behav. 1999;63(1):39-43.

Stevens CW. Relative analgesic potency of mu, delta and kappa opioids after spinal administration in amphibians. J Pharmacol Exp Ther. 1996;276(2):440-8.